An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Global analysis of TDP-43 interacting proteins reveals strong association with RNA splicing and translation machinery.

Freibaum BD, Chitta RK, High AA, Taylor JP

TDP-43 is a highly conserved and ubiquitously expressed member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family of proteins. Recently, TDP-43 was shown to be a major disease protein in the ubiquitinated inclusions characteristic of most cases of amyotrophic lateral sclerosis (ALS), tau-negative frontotemporal lobar degeneration (FTLD), and inclusion body myopathy. In these diseases, TDP-43 is redistributed from its predominantly nuclear ... [more]

J. Proteome Res. Feb. 05, 2010; 9(2);1104-20 [Pubmed: 20020773]

Quantitative Score

2.9 [Confidence Score]

Throughput

High Throughput

Additional Notes

FLAG-tagged interactor A
score=co-IP spectra/negative control spectra

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SYNCRIP TARDBP	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2012)	High	0.899	BioGRID	744778

Curated By

BioGRID

Interaction Summary

TARDBP

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA]double-stranded DNA binding [IDA]identical protein binding [IPI]mRNA 3'-UTR binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]

Gene Ontology Cellular Component

SYNCRIP