An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Global analysis of TDP-43 interacting proteins reveals strong association with RNA splicing and translation machinery.

Freibaum BD, Chitta RK, High AA, Taylor JP

TDP-43 is a highly conserved and ubiquitously expressed member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family of proteins. Recently, TDP-43 was shown to be a major disease protein in the ubiquitinated inclusions characteristic of most cases of amyotrophic lateral sclerosis (ALS), tau-negative frontotemporal lobar degeneration (FTLD), and inclusion body myopathy. In these diseases, TDP-43 is redistributed from its predominantly nuclear ... [more]

J. Proteome Res. Feb. 05, 2010; 9(2);1104-20 [Pubmed: 20020773]

Quantitative Score

2.0 [Confidence Score]

Throughput

High Throughput

Additional Notes

FLAG-tagged interactor A
score=co-IP spectra/negative control spectra

Curated By

BioGRID

Interaction Summary

TARDBP

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [IDA]double-stranded DNA binding [IDA]identical protein binding [IPI]mRNA 3'-UTR binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]

Gene Ontology Cellular Component

AGO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA 7-methylguanosine cap binding [IDA]endoribonuclease activity [EXP]endoribonuclease activity, cleaving siRNA-paired mRNA [IDA]poly(A) RNA binding [IDA]protein binding [IPI]siRNA binding [IDA]translation initiation factor activity [NAS]