BAIT

TRPV1

OTRPC1, TRPV1alpha, TRPV1beta, VR-1, Vr1, RP23-214D6.2
transient receptor potential cation channel, subfamily V, member 1
GO Process (33)
GO Function (13)
GO Component (11)
Mus musculus
PREY

PRKACA

PKCD, Pkaca
protein kinase, cAMP dependent, catalytic, alpha
GO Process (20)
GO Function (10)
GO Component (16)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Protein kinase A anchoring via AKAP150 is essential for TRPV1 modulation by forskolin and prostaglandin E2 in mouse sensory neurons.

Schnizler K, Shutov LP, Van Kanegan MJ, Merrill MA, Nichols B, McKnight GS, Strack S, Hell JW, Usachev YM

Phosphorylation-dependent modulation of the vanilloid receptor TRPV1 is one of the key mechanisms mediating the hyperalgesic effects of inflammatory mediators, such as prostaglandin E(2) (PGE(2)). However, little is known about the molecular organization of the TRPV1 phosphorylation complex and specifically about scaffolding proteins that position the protein kinase A (PKA) holoenzyme proximal to TRPV1 for effective and selective regulation of ... [more]

J. Neurosci. May. 07, 2008; 28(19);4904-17 [Pubmed: 18463244]

Throughput

  • Low Throughput

Curated By

  • BioGRID