An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

USP47 and C terminus of Hsp70-interacting protein (CHIP) antagonistically regulate katanin-p60-mediated axonal growth.

Yang SW, Oh KH, Park E, Chang HM, Park JM, Seong MW, Ka SH, Song WK, Park DE, Baas PW, Jeon YJ, Chung CH

Katanin is a heterodimeric enzyme that severs and disassembles microtubules. While the p60 subunit has the enzyme activity, the p80 subunit regulates the p60 activity. The microtubule-severing activity of katanin plays an essential role in axonal growth. However, the mechanisms by which neuronal cells regulate the expression of katanin-p60 remains unknown. Here we showed that USP47 and C terminus of ... [more]

J. Neurosci. Jul. 31, 2013; 33(31);12728-38 [Pubmed: 23904609]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

KATNA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [TAS]ATPase activity [ISO, TAS]dynein binding [ISO]microtubule binding [ISO]microtubule-severing ATPase activity [IBA, ISO]protein heterodimerization activity [ISO]ubiquitin-protein transferase activity [ISS]

Gene Ontology Cellular Component

RUFY3