BAIT
HECW2
NEDL2
HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
GO Process (1)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
ZP3
ZP3A, ZP3B, ZPC, Zp-3
zona pellucida glycoprotein 3 (sperm receptor)
GO Process (32)
GO Function (6)
GO Component (12)
Gene Ontology Biological Process
- binding of sperm to zona pellucida [IDA, IGI, IMP, TAS]
- blastocyst formation [ISS]
- calcium ion transmembrane transport [IDA]
- egg coat formation [ISS]
- humoral immune response mediated by circulating immunoglobulin [ISS]
- intracellular protein transport [ISS]
- intracellular signal transduction [IMP]
- manganese ion transmembrane transport [ISS]
- manganese ion transport [ISS]
- multicellular organism reproduction [TAS]
- negative regulation of binding of sperm to zona pellucida [IDA]
- negative regulation of transcription, DNA-templated [ISS]
- oocyte development [ISS]
- phosphatidylinositol-mediated signaling [ISS]
- positive regulation of T cell proliferation [ISS]
- positive regulation of acrosomal vesicle exocytosis [IDA, IMP]
- positive regulation of acrosome reaction [IDA]
- positive regulation of antral ovarian follicle growth [ISS]
- positive regulation of calcium ion import [IDA]
- positive regulation of humoral immune response [IDA]
- positive regulation of inflammatory response [ISS]
- positive regulation of interferon-gamma production [ISS]
- positive regulation of interleukin-4 production [ISS]
- positive regulation of leukocyte migration [ISS]
- positive regulation of ovarian follicle development [ISS]
- positive regulation of phosphatidylinositol biosynthetic process [ISS]
- positive regulation of protein kinase B signaling [ISS]
- positive regulation of protein kinase activity [IDA]
- positive regulation of transcription, DNA-templated [ISS]
- positive regulation of type IV hypersensitivity [ISS]
- protein kinase C signaling [IMP]
- single fertilization [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
HECT Type Ubiquitin Ligase NEDL2 is Degraded by APC/C-Cdh1 and its Tight Regulation Maintains the Metaphase to Anaphase Transition.
NEDD4-like ubiquitin ligase 2 (NEDL2) is a HECT type ubiquitin ligase. NEDL2 enhances p73 transcriptional activity and degrades ATR kinase in lamin misexpressed cells. Compared to the important functions of other HECT type ubiquitin ligase, there is less study concerning the function and regulation of NEDL2. Using primary antibody immunoprecipitation and mass spectrometry (IP/MS), we identify a list of potential ... [more]
J. Biol. Chem. Oct. 25, 2013; 0(0); [Pubmed: 24163370]
Throughput
- High Throughput
Curated By
- BioGRID