Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The UbL protein UBTD1 stably interacts with the UBE2D family of E2 ubiquitin conjugating enzymes.

Uhler JP, Spahr H, Farge G, Clavel S, Larsson NG, Falkenberg M, Samuelsson T, Gustafsson CM

UBTD1 is a previously uncharacterized ubiquitin-like (UbL) domain containing protein with high homology to the mitochondrial Dc-UbP/UBTD2 protein. Here we show that UBTD1 and UBTD2 belong to a family of proteins that is conserved through evolution and found in metazoa, funghi, and plants. To gain further insight into the function of UBTD1, we screened for interacting proteins. In a yeast-2-hybrid ... [more]

Biochem. Biophys. Res. Commun. Nov. 06, 2013; 0(0); [Pubmed: 24211586]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

UBTD1

PFKM

Gene Ontology Biological Process

Gene Ontology Molecular Function

6-phosphofructokinase activity [IDA, IMP, ISS]ATP binding [IDA]fructose binding [IDA]identical protein binding [IPI]kinase binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

Two-hybrid

Publication

The UbL protein UBTD1 stably interacts with the UBE2D family of E2 ubiquitin conjugating enzymes.

Throughput

Curated By

6-phosphofructokinase activity [IDA, IMP, ISS]
ATP binding [IDA]
fructose binding [IDA]
identical protein binding [IPI]
kinase binding [IPI]
protein C-terminus binding [IPI]
protein binding [IPI]