ATG3
Gene Ontology Biological Process
Gene Ontology Molecular Function
ATG7
Gene Ontology Biological Process
- C-terminal protein lipidation [IBA]
- adult walking behavior [IMP]
- autophagy [IMP]
- cardiac muscle cell development [IMP]
- cellular amino acid metabolic process [IMP]
- cellular response to hyperoxia [ISO]
- cellular response to nitrogen starvation [IBA]
- cellular response to starvation [ISO]
- central nervous system neuron axonogenesis [IMP]
- cerebellar Purkinje cell layer development [IMP]
- cerebral cortex development [IMP]
- late nucleophagy [IBA]
- liver development [IMP]
- macroautophagy [IBA]
- membrane organization [IMP]
- mitochondrion degradation [IBA]
- mitochondrion organization [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of histone H4-K16 acetylation [IMP]
- negative stranded viral RNA replication [IMP]
- neurological system process [IMP]
- neuron projection development [IMP]
- organelle organization [IMP]
- piecemeal microautophagy of nucleus [IBA]
- positive regulation of apoptotic process [ISO]
- positive regulation of autophagy [ISO]
- positive regulation of macroautophagy [ISO]
- positive regulation of mucus secretion [IMP]
- positive regulation of protein catabolic process [ISO]
- positive regulation of protein modification process [IDA, ISO]
- post-embryonic development [IMP]
- protein catabolic process [IMP]
- protein lipidation [ISO]
- protein modification by small protein conjugation [IMP]
- pyramidal neuron development [IMP]
- regulation of protein ubiquitination [IMP]
- response to starvation [IMP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Structural basis of ATG3 recognition by the autophagic ubiquitin-like protein ATG12.
The autophagic ubiquitin-like protein (ublp) autophagy-related (ATG)12 is a component of the ATG12∼ATG5-ATG16L1 E3 complex that promotes lipid conjugation of members of the LC3 ublp family. A role of ATG12 in the E3 complex is to recruit the E2 enzyme ATG3. Here we report the identification of the ATG12 binding sequence in the flexible region of human ATG3 and the ... [more]
Throughput
- Low Throughput
Additional Notes
- #LPPI
- Likely protein-protein interaction
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATG7 ATG3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 728515 | |
| ATG3 ATG7 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 728516 | |
| ATG3 ATG7 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.887 | BioGRID | 2667020 | |
| ATG7 ATG3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID