Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Hypoxia-Induced Inhibition of ENaC in the Lung: Role of Nedd4-2 and the Ubiquitin-Proteasome Pathway.

Gille T, Randrianarison-Pellan N, Goolaerts A, Dard N, Uzunhan Y, Ferrary E, Hummler E, Clerici C, Planes C

Transepithelial sodium transport via alveolar epithelial Na(+) channels (ENaC) and Na(+),K(+)-ATPase constitutes the driving force for removal of alveolar edema fluid. Alveolar hypoxia associated with pulmonary edema may impair ENaC activity and alveolar Na(+) absorption through a decrease of ENaC subunit expression at the apical membrane of alveolar epithelial cells (AEC). Here, we investigated the mechanism(s) involved in this process ... [more]

Am. J. Respir. Cell Mol. Biol. Oct. 04, 2013; 0(0); [Pubmed: 24093724]

Throughput

  • Low Throughput

Additional Notes

  • likely ubiquitin conjugate

Curated By

  • BioGRID