BAIT

IL2RA

CD25, IDDM10, IL2R, TCGFR, p55, RP11-536K7.1
interleukin 2 receptor, alpha
GO Process (6)
GO Function (1)
GO Component (1)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A hydrophobic amino acid cluster inserted into the C-terminus of a recycling cell surface receptor functions as an endosomal sorting signal.

Amano Y, Yoshino K, Kojima K, Takeshita T

Cell surface receptors ubiquitylated after ligand stimulation are internalized and delivered to the lysosomal pathway for degradation. Ubiquitylated receptors are captured by ESCRT protein complexes that sort them to the lysosomal pathway. Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a component of endosomal sorting complexes required for transport (ESCRT)-0 that recognizes ubiquitin attached to receptors, indicating that it functions ... [more]

Biochem. Biophys. Res. Commun. Nov. 08, 2013; 441(1);164-8 [Pubmed: 24134837]

Throughput

  • Low Throughput

Curated By

  • BioGRID