BAIT

GIF3

A_TM018A10.22, A_TM018A10_22, GRF1-interacting factor 3, T18A10.15, T18A10_15, AT4G00850
GRF1-interacting factor 3
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

GRF4

AtGRF4, growth-regulating factor 4, AT3G52910
growth-regulating factor 4
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Molecular mechanism of miR396 mediating pistil development in Arabidopsis thaliana.

Liang G, He H, Li Y, Wang F, Yu D

The precise control of gene regulation, and hence, correct spatio-temporal tissue patterning, is crucial for plant development. Plant microRNAs can constrain the expression of their target genes at post-transcriptional levels. Recently, miR396 has been characterized to regulated leaf development by mediating cleavage of its GRF targets. miR396 is also preferentially expressed in flowers. However, its function in flower development is ... [more]

Plant Physiol. Nov. 27, 2013; 0(0); [Pubmed: 24285851]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GIF3 GRF4
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
-
GRF4 GIF3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
GRF4 GIF3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BAR
2504338

Curated By

  • BioGRID