PREY

STUB1

CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7, UBOX1, LA16c-313D11.6
STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
GO Process (15)
GO Function (15)
GO Component (10)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Opposing Actions of Heat Shock Protein 90 and 70 Regulate Nicotinamide Adenine Dinucleotide Phosphate Oxidase Stability and Reactive Oxygen Species Production.

Chen F, Yu Y, Qian J, Wang Y, Cheng B, Dimitropoulou C, Patel V, Chadli A, Rudic RD, Stepp DW, Catravas JD, Fulton DJ

OBJECTIVE: Excessive reactive oxygen species contribute to vascular dysfunction. We have previously shown that heat shock protein (Hsp90) inhibitors potently suppress Nox 1 to 3 and 5, and the goals of this study were to identify how molecular chaperones regulate Nox function. METHODS AND RESULTS: In vitro, protein expression of Nox 1 to 2, 5 was decreased by Hsp90 inhibitors ... [more]

Arterioscler. Thromb. Vasc. Biol. Sep. 27, 2012; 0(0); [Pubmed: 23023377]

Throughput

  • Low Throughput

Curated By

  • BioGRID