An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Analysis of the role of calmodulin binding and sequestration in neuromodulin (GAP-43) function.

Gamby C, Waage MC, Allen RG, Baizer L

We demonstrated previously that forced expression of the neuronal phosphoprotein neuromodulin (also known as GAP-43, F1, B-50, and p57) in mouse anterior pituitary AtT-20 cells enhances depolarization-mediated secretion and alters cellular morphology. Here we analyze the role of calmodulin binding by neuromodulin in these responses. In cells expressing wild-type neuromodulin, a complex with calmodulin that is sensitive to intracellular calcium ... [more]

J. Biol. Chem. Oct. 25, 1996; 271(43);26698-705 [Pubmed: 8900147]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

GAP43

Gene Ontology Biological Process

Gene Ontology Molecular Function

calmodulin binding [IBA]

Gene Ontology Cellular Component

CALM1