Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A Y2H-seq approach defines the human protein methyltransferase interactome.

Weimann M, Grossmann A, Woodsmith J, Oezkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U

To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and ... [more]

Nat. Methods Apr. 01, 2013; 10(4);339-42 [Pubmed: 23455924]

Throughput

  • High Throughput

Additional Notes

  • Hit identified by mass spec

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HESX1 PRMT6
Affinity Capture-Luminescence
Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

High-BioGRID
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Curated By

  • BioGRID