BAIT
ISL1
ISLET1, Isl-1
ISL LIM homeobox 1
GO Process (36)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
- atrial septum morphogenesis [ISS]
- cardiac cell fate determination [IDA]
- cardiac right ventricle morphogenesis [ISS]
- cellular response to glucocorticoid stimulus [ISS]
- endocardial cushion morphogenesis [ISS]
- innervation [ISS]
- mesenchymal cell differentiation [ISS]
- negative regulation of inflammatory response [ISS]
- negative regulation of intracellular estrogen receptor signaling pathway [ISS]
- negative regulation of neuron apoptotic process [ISS]
- negative regulation of protein homodimerization activity [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- neuron fate specification [ISS]
- outflow tract morphogenesis [ISS]
- outflow tract septum morphogenesis [ISS]
- pancreas development [ISS]
- peripheral nervous system neuron axonogenesis [ISS]
- pharyngeal system development [ISS]
- positive regulation of angiogenesis [ISS]
- positive regulation of granulocyte colony-stimulating factor production [ISS]
- positive regulation of granulocyte macrophage colony-stimulating factor production [ISS]
- positive regulation of insulin secretion [IC]
- positive regulation of interferon-gamma production [ISS]
- positive regulation of interleukin-1 alpha production [ISS]
- positive regulation of interleukin-1 beta production [ISS]
- positive regulation of interleukin-12 production [ISS]
- positive regulation of interleukin-6 production [ISS]
- positive regulation of macrophage colony-stimulating factor production [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of tumor necrosis factor production [ISS]
- positive regulation vascular endothelial growth factor production [ISS]
- secondary heart field specification [IMP]
- sensory system development [ISS]
- spinal cord motor neuron differentiation [ISS]
- trigeminal nerve development [ISS]
- ventricular cardiac muscle tissue morphogenesis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
KDM1A
AOF2, BHC110, KDM1, LSD1, RP1-184J9.1
lysine (K)-specific demethylase 1A
GO Process (15)
GO Function (16)
GO Component (4)
Gene Ontology Biological Process
- blood coagulation [TAS]
- histone H3-K4 demethylation [IDA]
- histone H3-K9 demethylation [IDA]
- muscle cell development [ISS]
- negative regulation of DNA binding [IC]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [IMP]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- negative regulation of protein binding [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISS]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein demethylation [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
Gene Ontology Molecular Function- MRF binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- demethylase activity [IMP]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA]
- histone demethylase activity [IDA]
- histone demethylase activity (H3-K4 specific) [IDA]
- histone demethylase activity (H3-K9 specific) [IDA]
- histone demethylase activity (H3-dimethyl-K4 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP]
- oxidoreductase activity [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- transcription factor binding [IDA]
- transcription regulatory region DNA binding [ISS]
- MRF binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- demethylase activity [IMP]
- enzyme binding [IPI]
- flavin adenine dinucleotide binding [IDA]
- histone demethylase activity [IDA]
- histone demethylase activity (H3-K4 specific) [IDA]
- histone demethylase activity (H3-K9 specific) [IDA]
- histone demethylase activity (H3-dimethyl-K4 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP]
- oxidoreductase activity [IDA]
- p53 binding [IPI]
- protein binding [IPI]
- transcription factor binding [IDA]
- transcription regulatory region DNA binding [ISS]
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A Y2H-seq approach defines the human protein methyltransferase interactome.
To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and ... [more]
Nat. Methods Apr. 01, 2013; 10(4);339-42 [Pubmed: 23455924]
Throughput
- High Throughput
Additional Notes
- Hit identified by mass spec
Curated By
- BioGRID