BAIT
PNKP
EIEE10, MCSZ, PNK
polynucleotide kinase 3'-phosphatase
GO Process (10)
GO Function (9)
GO Component (4)
Gene Ontology Biological Process
- DNA damage response, detection of DNA damage [IDA, NAS]
- DNA repair [IGI]
- DNA-dependent DNA replication [NAS]
- dephosphorylation [IDA]
- nucleic acid phosphodiester bond hydrolysis [NAS]
- nucleotide phosphorylation [IDA]
- nucleotide-excision repair, DNA damage removal [NAS]
- polynucleotide 3' dephosphorylation [IDA]
- response to oxidative stress [IDA]
- response to radiation [NAS, TAS]
Gene Ontology Molecular Function- ATP binding [NAS]
- ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity [IDA]
- damaged DNA binding [NAS]
- double-stranded DNA binding [TAS]
- endonuclease activity [NAS]
- nucleotide kinase activity [IDA]
- polynucleotide 3'-phosphatase activity [IDA]
- protein binding [IPI]
- purine nucleotide binding [NAS]
- ATP binding [NAS]
- ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity [IDA]
- damaged DNA binding [NAS]
- double-stranded DNA binding [TAS]
- endonuclease activity [NAS]
- nucleotide kinase activity [IDA]
- polynucleotide 3'-phosphatase activity [IDA]
- protein binding [IPI]
- purine nucleotide binding [NAS]
Homo sapiens
PREY
SUV39H1
H3-K9-HMTase 1, KMT1A, MG44, SUV39H
suppressor of variegation 3-9 homolog 1 (Drosophila)
GO Process (9)
GO Function (8)
GO Component (5)
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IDA]
- cellular response to hypoxia [IDA]
- chromatin organization [TAS]
- chromatin silencing at rDNA [IDA]
- histone H3-K9 dimethylation [ISS]
- histone H3-K9 trimethylation [ISS]
- negative regulation of circadian rhythm [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [ISS]
Gene Ontology Molecular Function- S-adenosylmethionine-dependent methyltransferase activity [IDA]
- chromatin binding [TAS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K9 specific) [IDA]
- histone-lysine N-methyltransferase activity [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription regulatory region sequence-specific DNA binding [ISS]
- S-adenosylmethionine-dependent methyltransferase activity [IDA]
- chromatin binding [TAS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K9 specific) [IDA]
- histone-lysine N-methyltransferase activity [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription regulatory region sequence-specific DNA binding [ISS]
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A Y2H-seq approach defines the human protein methyltransferase interactome.
To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and ... [more]
Nat. Methods Apr. 01, 2013; 10(4);339-42 [Pubmed: 23455924]
Throughput
- High Throughput
Additional Notes
- Hit identified by mass spec
Curated By
- BioGRID