PSMC1
Gene Ontology Biological Process
- ATP catabolic process [ISS]
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- RNA metabolic process [TAS]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- apoptotic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- mitotic cell cycle [TAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- protein polyubiquitination [TAS]
- regulation of apoptotic process [TAS]
- regulation of cellular amino acid metabolic process [TAS]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- small molecule metabolic process [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SUV39H1
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IDA]
- cellular response to hypoxia [IDA]
- chromatin organization [TAS]
- chromatin silencing at rDNA [IDA]
- histone H3-K9 dimethylation [ISS]
- histone H3-K9 trimethylation [ISS]
- negative regulation of circadian rhythm [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [ISS]
Gene Ontology Molecular Function- S-adenosylmethionine-dependent methyltransferase activity [IDA]
- chromatin binding [TAS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K9 specific) [IDA]
- histone-lysine N-methyltransferase activity [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription regulatory region sequence-specific DNA binding [ISS]
- S-adenosylmethionine-dependent methyltransferase activity [IDA]
- chromatin binding [TAS]
- histone methyltransferase activity [IDA]
- histone methyltransferase activity (H3-K9 specific) [IDA]
- histone-lysine N-methyltransferase activity [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- transcription regulatory region sequence-specific DNA binding [ISS]
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A Y2H-seq approach defines the human protein methyltransferase interactome.
To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and ... [more]
Throughput
- High Throughput
Additional Notes
- Hit identified by mass spec
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PSMC1 SUV39H1 | Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag. | High | - | BioGRID | - |
Curated By
- BioGRID