BAIT
VHL
HRCA1, RCA1, VHL1, pVHL
von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
GO Process (13)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- cell morphogenesis [NAS]
- cellular response to hypoxia [TAS]
- negative regulation of apoptotic process [NAS]
- negative regulation of cell proliferation [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- negative regulation of transcription from RNA polymerase II promoter in response to hypoxia [IDA]
- positive regulation of cell differentiation [NAS]
- positive regulation of transcription, DNA-templated [IMP]
- protein stabilization [NAS]
- protein ubiquitination [IDA, IMP]
- proteolysis [TAS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- regulation of transcription, DNA-templated [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PRMT8
HRMT1L3, HRMT1L4
protein arginine methyltransferase 8
GO Process (7)
GO Function (9)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function- S-adenosylmethionine-dependent methyltransferase activity [IDA]
- histone methyltransferase activity (H4-R3 specific) [IBA]
- histone-arginine N-methyltransferase activity [IDA]
- identical protein binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA]
- protein-arginine omega-N asymmetric methyltransferase activity [IDA]
- protein-arginine omega-N monomethyltransferase activity [IDA]
- S-adenosylmethionine-dependent methyltransferase activity [IDA]
- histone methyltransferase activity (H4-R3 specific) [IBA]
- histone-arginine N-methyltransferase activity [IDA]
- identical protein binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA]
- protein-arginine omega-N asymmetric methyltransferase activity [IDA]
- protein-arginine omega-N monomethyltransferase activity [IDA]
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A Y2H-seq approach defines the human protein methyltransferase interactome.
To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and ... [more]
Nat. Methods Apr. 01, 2013; 10(4);339-42 [Pubmed: 23455924]
Throughput
- High Throughput
Additional Notes
- Hit identified by mass spec
Curated By
- BioGRID