MAPKAPK2
Gene Ontology Biological Process
- 3'-UTR-mediated mRNA stabilization [IDA]
- G2 DNA damage checkpoint [IMP]
- MAPK cascade [TAS]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- RNA metabolic process [TAS]
- Ras protein signal transduction [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- activation of MAPK activity [TAS]
- arachidonic acid metabolic process [TAS]
- cellular response to DNA damage stimulus [IMP]
- cellular response to vascular endothelial growth factor stimulus [IMP]
- gene expression [TAS]
- inflammatory response [ISS]
- innate immune response [TAS]
- leukotriene metabolic process [TAS]
- mRNA metabolic process [TAS]
- macropinocytosis [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-serine phosphorylation [IDA]
- protein phosphorylation [TAS]
- regulation of interleukin-6 production [ISS]
- regulation of tumor necrosis factor production [IDA]
- response to cytokine [IDA]
- response to lipopolysaccharide [ISS]
- response to stress [IDA]
- small molecule metabolic process [TAS]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [ISS, TAS]
- vascular endothelial growth factor receptor signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UBE2J1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Endoplasmic reticulum-associated ubiquitin conjugating enzyme- Ube2j1 is a novel substrate of MAPKAP kinase-2 involved in MK2-mediated TNFα production.
The p38 MAPK/MK2 signalling pathway is a major regulator of stress- and cytokine-induced gene expression at the transcriptional and post-transcriptional level. By phospho-proteomics we identified the ER-associated ubiquitin-conjugating enzyme Ube2j1 as a potential substrate of MK2. We demonstrate that Ube2j1 is phosphorylated in a cytokine-, cytosolic stress- and LPS-induced manner. The cytosolic stress-induced phosphorylation of Ube2j1 proceeds at serine 184, ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID