An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The Non-JAZ TIFY Protein TIFY8 from Arabidopsis thaliana Is a Transcriptional Repressor.

Cuellar Perez A, Nagels Durand A, Vanden Bossche R, De Clercq R, Persiau G, Van Wees SC, Pieterse CM, Gevaert K, De Jaeger G, Goossens A, Pauwels L

Jasmonate (JA) signalling is mediated by the JASMONATE-ZIM DOMAIN (JAZ) repressor proteins, which are degraded upon JA perception to release downstream responses. The ZIM protein domain is characteristic of the larger TIFY protein family. It is currently unknown if the atypical member TIFY8 is involved in JA signalling. Here we show that the TIFY8 ZIM domain is functional and mediated ... [more]

PLoS ONE Jan. 15, 2014; 9(1);e84891 [Pubmed: 24416306]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NINJA TIFY8	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Altmann M (2020)	High	-	BioGRID	-
NINJA TIFY8	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Cuellar Perez A (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TIFY8

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

NINJA

Gene Ontology Biological Process