BAIT

SUGT1

SGT1, RP11-93H24.2
SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
UPS Project
GO Process (3)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

PMF1

RP11-54H19.4
polyamine-modulated factor 1
GO Process (3)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Plk1 phosphorylates Sgt1 at the kinetochores to promote timely kinetochore-microtubule attachment.

Liu XS, Song B, Tang J, Liu W, Kuang S, Liu X

Accurate chromosome segregation during cell division maintains genomic integrity and requires the proper establishment of kinetochore-microtubule attachment in mitosis. As a key regulator of mitosis, Polo-like kinase 1 (Plk1) is essential for this attachment process, but the molecular mechanism remains elusive. Here we identify Sgt1, a cochaperone for Hsp90, as a novel Plk1 substrate during mitosis. We show that Sgt1 ... [more]

Mol. Cell. Biol. Oct. 01, 2012; 32(19);4053-67 [Pubmed: 22869522]

Throughput

  • Low Throughput

Ontology Terms

  • hek-293t cell (BTO:0002181) [nocodazole (CHEBI:34892)]

Additional Notes

  • figure 9.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SUGT1 PMF1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID