BAIT

PPH3

phosphoprotein phosphatase PP4 catalytic subunit PPH3, L000001471, YDR075W
Catalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
Saccharomyces cerevisiae (S288c)
PREY

XRS2

L000002489, YDR369C
Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Phosphatase Complex Pph3/Psy2 Is Involved in Regulation of Efficient Non-Homologous End-Joining Pathway in the Yeast Saccharomyces cerevisiae.

Omidi K, Hooshyar M, Jessulat M, Samanfar B, Sanders M, Burnside D, Pitre S, Schoenrock A, Xu J, Babu M, Golshani A

One of the main mechanisms for double stranded DNA break (DSB) repair is through the non-homologous end-joining (NHEJ) pathway. Using plasmid and chromosomal repair assays, we showed that deletion mutant strains for interacting proteins Pph3p and Psy2p had reduced efficiencies in NHEJ. We further observed that this activity of Pph3p and Psy2p appeared linked to cell cycle Rad53p and Chk1p ... [more]

PLoS ONE Feb. 06, 2014; 9(1);e87248 [Pubmed: 24498054]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)
  • phenotype: resistance to chemicals (APO:0000087)

Additional Notes

  • when DNA damage was induced, overexpression of either PPH3 or PSY2 formed SDL interactions with gene deletion strains for each of the three members of MRX complex MRE11, RAD50 and XRS2 in a synthetic dosage lethal screen

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PPH3 XRS2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.226BioGRID
2094180
PPH3 XRS2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
927737

Curated By

  • BioGRID