BAIT

PSMG3

C7orf48, PAC3
proteasome (prosome, macropain) assembly chaperone 3
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

PSMB4

HN3, HsN3, PROS-26, PROS26
proteasome (prosome, macropain) subunit, beta type, 4
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Cooperation of multiple chaperones required for the assembly of mammalian 20S proteasomes.

Hirano Y, Hayashi H, Iemura S, Hendil KB, Niwa S, Kishimoto T, Kasahara M, Natsume T, Tanaka K, Murata S

The 20S proteasome is a catalytic core of the 26S proteasome, a central enzyme in the degradation of ubiquitin-conjugated proteins. It is composed of 14 distinct gene products that form four stacked rings of seven subunits each, alpha(1-7)beta(1-7)beta(1-7)alpha(1-7). It is reported that the biogenesis of mammalian 20S proteasomes is assisted by proteasome-specific chaperones, named PAC1, PAC2, and hUmp1, but the ... [more]

Mol. Cell Dec. 28, 2006; 24(6);977-84 [Pubmed: 17189198]

Throughput

  • Low Throughput

Curated By

  • BioGRID