HIST2H2AC
Gene Ontology Cellular Component
USP15
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- histone H2B conserved C-terminal lysine deubiquitination [IDA]
- monoubiquitinated protein deubiquitination [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- pathway-restricted SMAD protein phosphorylation [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA]
- regulation of proteasomal protein catabolic process [IBA]
- transforming growth factor beta receptor signaling pathway [IDA, IMP, TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination.
Post-translational modifications of histone proteins produce dynamic signals that regulate the structure and function of chromatin. Mono-ubiquitination of H2B in the histone tail (at K123 in yeast or K120 in humans) is a conserved modification that has been implicated in the regulation of transcription, replication, and DNA repair processes. In a search for direct effectors of ubH2B, we identified a ... [more]
Throughput
- Low Throughput
Additional Notes
- ubiquitinated H2A/H2B dimers, where one histone of the pair is ubiquitinated and the other is FLAG-tagged
Curated By
- BioGRID