BAIT

CAMLG

Caml
calcium modulating ligand
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Rattus norvegicus
PREY

TMUB1

Hops
transmembrane and ubiquitin-like domain containing 1
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

IL-6 induction of hepatocyte proliferation through the Tmub1-regulated gene pathway.

Liu M, Liu H, Wang X, Chen P, Chen H

The expression of transmembrane and ubiquitin-like domain containing 1 (Tmub1) is upregulated during liver regeneration, however, the function and underlying molecular mechanisms responsible for Tmub1 action remain to be determined. This study utilized BRL-3A rat liver cells for Tmub1 shRNA lentivirus infection and IL-6 stimulation. Semi-quantitative RT-PCR and western blot analysis were used to detect mRNA and protein expression levels, ... [more]

Int. J. Mol. Med. Jun. 01, 2012; 29(6);1106-12 [Pubmed: 22426572]

Throughput

  • Low Throughput

Curated By

  • BioGRID