PREY

KCNAB2

potassium voltage-gated channel, shaker-related subfamily, beta member 2
GO Process (2)
GO Function (1)
GO Component (2)
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Modulation of delayed rectifier potassium channel by protein kinase C zeta-containing signaling complex in pheochromocytoma cells.

Kim Y, Uhm DY, Shin J, Chung S

Voltage-dependent delayed rectifier K(+) (Kv) channels are fundamental components in the regulation of neuronal excitability. We found that nerve growth factor (NGF) treatment of PC12 cells induced a hyperpolarizing shift of the Kv current activation curve by about 15 mV. This effect was similar to the effect of the modulatory subunit, Kv beta, on the cloned Kv channel, and required ... [more]

Neuroscience Apr. 06, 2004; 125(2);359-68 [Pubmed: 15062979]

Throughput

  • Low Throughput

Curated By

  • BioGRID