An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress.

Zhang L, Chen X, Sharma P, Moon M, Sheftel AD, Dawood F, Nghiem MP, Wu J, Li RK, Gramolini AO, Sorensen PH, Penninger JM, Brumell JH, Liu PP

The HECT E3 ubiquitin ligase HACE1 is a tumour suppressor known to regulate Rac1 activity under stress conditions. HACE1 is increased in the serum of patients with heart failure. Here we show that HACE1 protects the heart under pressure stress by controlling protein degradation. Hace1 deficiency in mice results in accelerated heart failure and increased mortality under haemodynamic stress. Hearts ... [more]

Nat Commun Mar. 13, 2014; 5(0);3430 [Pubmed: 24614889]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

HACE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [IDA]Rac GTPase binding [IPI]protein binding [IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

CORO1C