BAIT

SNC1

BAL, BALL, CONSTITUTIVE 1, DL4475C, FCAALL.51, SUPPRESSOR OF NPR1-1, AT4G16890
protein SUPPRESSOR OF npr1-1, CONSTITUTIVE 1
Arabidopsis thaliana (Columbia)
PREY

AT5G15400

T20K14.10, T20K14_10
putative ubiquitin conjugation factor E4
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

An e4 ligase facilitates polyubiquitination of plant immune receptor resistance proteins in Arabidopsis.

Huang Y, Minaker S, Roth C, Huang S, Hieter P, Lipka V, Wiermer M, Li X

Proteins with nucleotide binding and leucine-rich repeat domains (NLRs) serve as immune receptors in animals and plants that recognize pathogens and activate downstream defense responses. As high accumulation of NLRs can result in unwarranted autoimmune responses, their cellular concentrations must be tightly regulated. However, the molecular mechanisms of this process are poorly detailed. The F-box protein Constitutive expressor of PR ... [more]

Plant Cell Jan. 01, 2014; 26(1);485-96 [Pubmed: 24449689]

Throughput

  • Low Throughput

Curated By

  • BioGRID