RPA1
Gene Ontology Biological Process
- DNA recombinase assembly [TAS]
- DNA recombination [TAS]
- DNA repair [TAS]
- DNA replication [IMP, TAS]
- DNA strand elongation involved in DNA replication [TAS]
- DNA-dependent DNA replication [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- base-excision repair [IDA]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [IMP, TAS]
- mismatch repair [IMP]
- mitotic cell cycle [TAS]
- nucleotide-excision repair [IMP, TAS]
- nucleotide-excision repair, DNA damage removal [TAS]
- nucleotide-excision repair, DNA gap filling [TAS]
- telomere maintenance [IMP, TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MSH2
Gene Ontology Biological Process
- ATP catabolic process [IBA, IDA]
- B cell differentiation [ISS]
- B cell mediated immunity [ISS]
- DNA repair [IDA]
- double-strand break repair [IBA]
- intra-S DNA damage checkpoint [IBA]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IBA]
- isotype switching [IBA, ISS]
- maintenance of DNA repeat elements [IMP]
- male gonad development [ISS]
- meiotic gene conversion [IBA]
- meiotic mismatch repair [IBA]
- mismatch repair [IDA, IGI]
- negative regulation of DNA recombination [IDA, ISS]
- negative regulation of neuron apoptotic process [ISS]
- negative regulation of reciprocal meiotic recombination [IBA]
- positive regulation of helicase activity [IDA]
- postreplication repair [IDA]
- response to UV-B [IBA, ISS]
- response to X-ray [IBA, ISS]
- somatic hypermutation of immunoglobulin genes [IBA]
- somatic recombination of immunoglobulin gene segments [ISS]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA]
- ATPase activity [IDA]
- DNA binding [IDA]
- DNA-dependent ATPase activity [IBA]
- MutLalpha complex binding [IDA]
- Y-form DNA binding [IBA]
- dinucleotide insertion or deletion binding [IDA]
- dinucleotide repeat insertion binding [IDA]
- double-strand/single-strand DNA junction binding [IBA]
- double-stranded DNA binding [IDA]
- enzyme binding [IPI]
- four-way junction DNA binding [IDA]
- guanine/thymine mispair binding [IDA, IMP]
- heteroduplex DNA loop binding [IBA]
- magnesium ion binding [IDA]
- mismatched DNA binding [IDA]
- oxidized purine DNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase binding [IPI]
- single guanine insertion binding [IDA]
- single thymine insertion binding [IDA]
- single-stranded DNA binding [IDA]
- ADP binding [IDA]
- ATP binding [IDA]
- ATPase activity [IDA]
- DNA binding [IDA]
- DNA-dependent ATPase activity [IBA]
- MutLalpha complex binding [IDA]
- Y-form DNA binding [IBA]
- dinucleotide insertion or deletion binding [IDA]
- dinucleotide repeat insertion binding [IDA]
- double-strand/single-strand DNA junction binding [IBA]
- double-stranded DNA binding [IDA]
- enzyme binding [IPI]
- four-way junction DNA binding [IDA]
- guanine/thymine mispair binding [IDA, IMP]
- heteroduplex DNA loop binding [IBA]
- magnesium ion binding [IDA]
- mismatched DNA binding [IDA]
- oxidized purine DNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase binding [IPI]
- single guanine insertion binding [IDA]
- single thymine insertion binding [IDA]
- single-stranded DNA binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.
PRP19 is a ubiquitin ligase involved in pre-mRNA splicing and the DNA damage response (DDR). Although the role for PRP19 in splicing is well characterized, its role in the DDR remains elusive. Through a proteomic screen for proteins that interact with RPA-coated single-stranded DNA (RPA-ssDNA), we identified PRP19 as a sensor of DNA damage. PRP19 directly binds RPA and localizes ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MSH2 RPA1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.5847 | BioGRID | 1268919 | |
| RPA1 MSH2 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3632005 |
Curated By
- BioGRID