An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Characterizing WW Domain Interactions of Tumor Suppressor WWOX Reveals its Association with Multiprotein Networks.

Abu-Odeh M, Bar-Mag T, Huang H, Kim T, Salah Z, Abdeen SK, Sudol M, Reichmann D, Sidhu S, Kim PM, Aqeilan RI

WW domains are small modules present in regulatory and signaling proteins that mediate specific protein-protein interactions. The WW domain-containing oxidoreductase (WWOX) encodes a 46-kDa tumor suppressor that contains two N-terminal WW domains and a central short-chain dehydrogenase/reductase domain. Based on its ligand recognition motifs, the WW domain family is classified into four groups. The largest one, to which WWOX belongs, ... [more]

J. Biol. Chem. Feb. 18, 2014; 0(0); [Pubmed: 24550385]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

WWOX

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription coactivator activity [ISS]coenzyme binding [TAS]cofactor binding [TAS]enzyme binding [IPI]oxidoreductase activity [NAS, TAS]protein binding [IPI]protein dimerization activity [TAS]

Gene Ontology Cellular Component

IQSEC1