BAIT

MARCH8

MARCH-VIII, MIR, RNF178, c-MIR, RP11-67C2.1
membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase
Human Papilloma Virus (HPV) Project
UPS Project
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

UBE2H

E2-20K, GID3, UBC8, UBCH, UBCH2
ubiquitin-conjugating enzyme E2H
UPS Project
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins.

Bartee E, Mansouri M, Hovey Nerenberg BT, Gouveia K, Frueh K

Poxviruses and gamma-2 herpesviruses share the K3 family of viral immune evasion proteins that inhibit the surface expression of glycoproteins such as major histocompatibility complex class I (MHC-I), B7.2, ICAM-1, and CD95(Fas). K3 family proteins contain an amino-terminal PHD/LAP or RING-CH domain followed by two transmembrane domains. To examine whether human homologues are functionally related to the viral immunoevasins, we ... [more]

J. Virol. Feb. 01, 2004; 78(3);1109-20 [Pubmed: 14722266]

Throughput

  • Low Throughput

Additional Notes

  • In vitro ubiquitination assay with MARCH ligases and UbcH2 E2

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MARCH8 UBE2H
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID