SSF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PWP1
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Ribosome assembly factors Pwp1 and Nop12 are important for folding of 5.8S rRNA during ribosome biogenesis in Saccharomyces cerevisiae.
Previous work from our lab suggests that a group of interdependent assembly factors (A3 factors) is necessary to create early, stable pre-ribosomes. Many of these proteins bind at or near ITS2, but in their absence ITS1 is not removed from rRNA, suggesting long-range communication between these two spacers. By comparing the non-essential assembly factors Nop12 and Pwp1 we show that ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PWP1 SSF1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1612 | BioGRID | 2442882 |
Curated By
- BioGRID