BAIT

CEBPA

DBPCEP
CCAAT/enhancer binding protein (C/EBP), alpha
GO Process (34)
GO Function (16)
GO Component (6)
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

HDAC3 interacts with sumoylated C/EBPα to negatively regulate the LXRα expression in rat hepatocytes.

Ren J, Li D, Li Y, Lan X, Zheng J, Wang X, Ma J, Lu S

The expression changes of liver X receptor alpha (LXRα), histone deacetylase 3 (HDAC3) and CCAAT/enhancer binding protein alpha (C/EBPα) were detected in liver tissues of our high-fat-diet E3 rat model. The aim of this study is to pinpoint the molecular mechanism of HDAC3 and C/EBPα to orchestrate LXRα expression in hepatocytes. We confirmed that LXRα and its target genes were ... [more]

Mol. Cell. Endocrinol. Jul. 15, 2013; 374(1);35-45 [Pubmed: 23639777]

Throughput

  • Low Throughput

Additional Notes

  • likely sumo conjugate

Curated By

  • BioGRID