PACS2
Gene Ontology Biological Process
Gene Ontology Cellular Component
BIRC3
Gene Ontology Biological Process
- MyD88-independent toll-like receptor signaling pathway [TAS]
- NIK/NF-kappaB signaling [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- cell surface receptor signaling pathway [TAS]
- inhibition of cysteine-type endopeptidase activity involved in apoptotic process [IBA]
- innate immune response [TAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of necroptotic process [IBA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [TAS]
- positive regulation of protein ubiquitination [IDA]
- protein ubiquitination [IDA]
- regulation of RIG-I signaling pathway [TAS]
- regulation of apoptotic process [IMP]
- regulation of cysteine-type endopeptidase activity [TAS]
- regulation of inflammatory response [TAS]
- regulation of innate immune response [TAS]
- regulation of necroptotic process [IMP]
- regulation of nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- regulation of toll-like receptor signaling pathway [TAS]
- spindle assembly involved in mitosis [IBA]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
Lysosomal membrane permeabilization is an essential step in TRAIL-induced apoptosis of liver cancer cell lines. TRAIL-induced lysosomal membrane permeabilization is mediated by the multifunctional sorting protein PACS-2 and repressed by the E3 ligases cIAP-1 and cIAP-2. Despite the opposing roles for PACS-2 and cIAPs in TRAIL-induced apoptosis, an interaction between these proteins has yet to be examined. Herein, we report ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BIRC3 PACS2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| BIRC3 PACS2 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 939022 |
Curated By
- BioGRID