A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Exo70E2 is essential for exocyst subunit recruitment and for EXPO formation in both plants and animals.

Ding Y, Wang J, Chun Lai JH, Ling Chan VH, Wang X, Cai Y, Tan X, Bao Y, Xia J, Robinson DG, Jiang L

In contrast to a single copy of Exo70 in yeast and mammals, the Arabidopsis genome contains 23 paralogs of Exo70 (AtExo70). Using AtExo70E2 and its GFP fusion as probes, we have recently identified a novel double-membrane organelle termed EXPO (exocyst-positive organelle) that mediates an unconventional protein secretion in plant cells. Here we further demonstrate that AtExo70E2 is essential for exocyst ... [more]

Mol. Biol. Cell Dec. 04, 2013; 0(0); [Pubmed: 24307681]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EXO70E2 SEC6	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Ding Y (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EXO70E2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

SEC6