PARP1
Gene Ontology Biological Process
- DNA damage response, detection of DNA damage [ISO]
- DNA metabolic process [IMP]
- DNA repair [TAS]
- base-excision repair [IMP]
- cellular response to insulin stimulus [ISO]
- cellular response to superoxide [IDA]
- double-strand break repair [IGI, ISO]
- positive regulation of SMAD protein import into nucleus [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription regulatory region DNA binding [ISO]
- protein ADP-ribosylation [IBA, ISO]
- protein autoprocessing [ISO]
- protein poly-ADP-ribosylation [ISO]
- regulation of growth rate [IMP]
- signal transduction involved in regulation of gene expression [ISO]
- telomere maintenance [IMP]
- transforming growth factor beta receptor signaling pathway [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
UHRF1
Gene Ontology Biological Process
- cell proliferation [TAS]
- histone monoubiquitination [IDA]
- histone ubiquitination [ISO]
- maintenance of DNA methylation [IMP, ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of cellular protein metabolic process [ISO]
- protein autoubiquitination [ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
Gene Ontology Molecular Function- core promoter proximal region sequence-specific DNA binding [ISO]
- hemi-methylated DNA-binding [IDA, ISO]
- histone binding [ISO]
- identical protein binding [IPI]
- methyl-CpG binding [ISO]
- methylated histone binding [IDA, ISO]
- nucleosomal histone binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase activity [IDA]
- ubiquitin-protein transferase activity [IDA, ISO]
- zinc ion binding [ISO]
- core promoter proximal region sequence-specific DNA binding [ISO]
- hemi-methylated DNA-binding [IDA, ISO]
- histone binding [ISO]
- identical protein binding [IPI]
- methyl-CpG binding [ISO]
- methylated histone binding [IDA, ISO]
- nucleosomal histone binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase activity [IDA]
- ubiquitin-protein transferase activity [IDA, ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Poly(ADP-ribose) polymerase 1 (PARP1) associates with E3 ubiquitine-protein ligase UHRF1 and modulates UHRF1 biological functions.
Poly(ADP-ribose) polymerase 1 (PARP1, also known as ARTD1) is an abundant nuclear enzyme that plays important roles in DNA repair, gene transcription and differentiation through the modulation of chromatin structure and function. In this work we identify a physical and functional poly(ADP-ribose) mediated interaction of PARP1 with the E3 ubiquitin ligase UHRF1 (also known as NP95, ICBP90) that influences two ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UHRF1 PARP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| UHRF1 PARP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID