An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS.

Varjosalo M, Sacco R, Stukalov A, van Drogen A, Planyavsky M, Hauri S, Aebersold R, Bennett KL, Colinge J, Gstaiger M, Superti-Furga G

The characterization of all protein complexes of human cells under defined physiological conditions using affinity purification-mass spectrometry (AP-MS) is a highly desirable step in the quest to understand the phenotypic effects of genomic information. However, such a challenging goal has not yet been achieved, as it requires reproducibility of the experimental workflow and high data consistency across different studies and ... [more]

Nat. Methods Apr. 01, 2013; 10(4);307-14 [Pubmed: 23455922]

Throughput

High Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007) [epithelial cell (CL:0000066)]

Additional Notes

exogenous expression of bait

Curated By

BioGRID

Interaction Summary

CSNK2A2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein N-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

SQSTM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH2 domain binding [IDA]identical protein binding [IPI]protein binding [IPI]protein kinase C binding [IPI]protein kinase binding [IDA]protein serine/threonine kinase activity [NAS]receptor tyrosine kinase binding [TAS]ubiquitin binding [IDA]