Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.


The Ku70 autoantigen interacts with p40phox in B lymphocytes.

Grandvaux N, Grizot S, Vignais PV, Dagher MC

Ku70, a regulatory component of the DNA-dependent protein kinase, was identified by a yeast two-hybrid screen of a B lymphocyte cDNA library as a partner of p40phox, a regulatory component of the O2--producing NADPH oxidase. Truncated constructs of p40phox and Ku70 were used to map the interacting sites. The 186 C-terminal amino acids (aa) of Ku70 were found to interact ... [more]

J. Cell. Sci. Feb. 01, 1999; 112(0);503-13 [Pubmed: 9914162]


  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.


Curated By

  • BioGRID