An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

TRIM65 regulates microRNA activity by ubiquitination of TNRC6.

Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME

MicroRNAs (miRNAs) are small evolutionarily conserved regulatory RNAs that modulate mRNA stability and translation in a wide range of cell types. MiRNAs are involved in a broad array of biological processes, including cellular proliferation, differentiation, and apoptosis. To identify previously unidentified regulators of miRNA, we initiated a systematic discovery-type proteomic analysis of the miRNA pathway interactome in human cells. Six ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 13, 2014; 111(19);6970-5 [Pubmed: 24778252]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRKRA NOP16	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9616	BioGRID	3144489

Curated By

BioGRID

Interaction Summary

PRKRA

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme activator activity [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [IPI]

Gene Ontology Cellular Component

NOP16