BAIT

DTX3L

BBAP
deltex 3 like, E3 ubiquitin ligase
GO Process (3)
GO Function (3)
GO Component (3)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The ubiquitin ligase Deltex-3L regulates endosomal sorting of the G protein-coupled receptor CXCR4.

Holleman J, Marchese A

G protein-coupled receptor (GPCR) sorting into the degradative pathway is important for limiting the duration and magnitude of signaling. Agonist activation of the GPCR CXCR4 induces its rapid ubiquitination and sorting to lysosomes via the endosomal sorting complex required for transport (ESCRT) pathway. We recently reported that ESCRT-0 ubiquitination is linked to the efficiency with which CXCR4 is sorted for ... [more]

Mol. Biol. Cell Apr. 30, 2014; 0(0); [Pubmed: 24790097]

Throughput

  • Low Throughput

Curated By

  • BioGRID