ADRB2
Gene Ontology Biological Process
- activation of adenylate cyclase activity [IDA]
- activation of transmembrane receptor protein tyrosine kinase activity [TAS]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [TAS]
- adrenergic receptor signaling pathway [IDA]
- cell surface receptor signaling pathway [TAS]
- desensitization of G-protein coupled receptor protein signaling pathway by arrestin [IDA]
- endosome to lysosome transport [TAS]
- positive regulation of MAPK cascade [IDA]
- positive regulation of protein ubiquitination [IMP]
- receptor-mediated endocytosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CCKBR
Gene Ontology Biological Process
- cell proliferation [TAS]
- cell surface receptor signaling pathway [NAS]
- cholecystokinin signaling pathway [TAS]
- digestion [TAS]
- feeding behavior [TAS]
- metabolic process [TAS]
- phospholipase C-activating G-protein coupled receptor signaling pathway [TAS]
- positive regulation of cell proliferation [IDA]
- positive regulation of cytosolic calcium ion concentration [IDA]
- regulation of phosphatidylinositol 3-kinase activity [TAS]
- sensory perception [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
CHIP-MYTH: A novel interactive proteomics method for the assessment of agonist-dependent interactions of the human β2-adrenergic receptor.
G-protein coupled receptors (GPCRs) are involved in a variety of disease processes and comprise major drug targets. However, the complexity of integral membrane proteins such as GPCRs makes the identification of their interacting partners and subsequent drug development challenging. A comprehensive understanding of GPCR protein interaction networks is needed to design effective therapeutic strategies to inhibit these drug targets. Here, ... [more]
Throughput
- High Throughput
Additional Notes
- MYTH split-ubiquitin system
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ADRB2 CCKBR | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2826909 |
Curated By
- BioGRID