ADRB2
Gene Ontology Biological Process
- activation of adenylate cyclase activity [IDA]
- activation of transmembrane receptor protein tyrosine kinase activity [TAS]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [TAS]
- adrenergic receptor signaling pathway [IDA]
- cell surface receptor signaling pathway [TAS]
- desensitization of G-protein coupled receptor protein signaling pathway by arrestin [IDA]
- endosome to lysosome transport [TAS]
- positive regulation of MAPK cascade [IDA]
- positive regulation of protein ubiquitination [IMP]
- receptor-mediated endocytosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
KCNE1
Gene Ontology Biological Process
- cardiac muscle cell action potential involved in contraction [IMP]
- cellular response to cAMP [IDA]
- membrane repolarization [IDA]
- membrane repolarization during action potential [IDA]
- membrane repolarization during cardiac muscle cell action potential [IDA, IMP]
- positive regulation of potassium ion transmembrane transport [IDA]
- potassium ion export [IDA]
- potassium ion transmembrane transport [IDA]
- protein N-linked glycosylation [IDA]
- protein O-linked glycosylation [IDA]
- regulation of delayed rectifier potassium channel activity [IDA]
- regulation of heart rate by cardiac conduction [IMP]
- regulation of potassium ion transmembrane transport [IDA]
- regulation of ventricular cardiac muscle cell membrane repolarization [IMP]
- sensory perception of sound [TAS]
- ventricular cardiac muscle cell action potential [IMP]
Gene Ontology Molecular Function- delayed rectifier potassium channel activity [IDA]
- potassium channel regulator activity [IDA]
- protein binding [IPI]
- telethonin binding [IPI]
- voltage-gated potassium channel activity [IDA]
- voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization [IDA]
- voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization [IMP]
- delayed rectifier potassium channel activity [IDA]
- potassium channel regulator activity [IDA]
- protein binding [IPI]
- telethonin binding [IPI]
- voltage-gated potassium channel activity [IDA]
- voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization [IDA]
- voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization [IMP]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
CHIP-MYTH: A novel interactive proteomics method for the assessment of agonist-dependent interactions of the human β2-adrenergic receptor.
G-protein coupled receptors (GPCRs) are involved in a variety of disease processes and comprise major drug targets. However, the complexity of integral membrane proteins such as GPCRs makes the identification of their interacting partners and subsequent drug development challenging. A comprehensive understanding of GPCR protein interaction networks is needed to design effective therapeutic strategies to inhibit these drug targets. Here, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ADRB2 KCNE1 | Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag. | High | - | BioGRID | - | |
| ADRB2 KCNE1 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | High | - | BioGRID | 942260 |
Curated By
- BioGRID