USP15
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- histone H2B conserved C-terminal lysine deubiquitination [IDA]
- monoubiquitinated protein deubiquitination [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- pathway-restricted SMAD protein phosphorylation [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA]
- regulation of proteasomal protein catabolic process [IBA]
- transforming growth factor beta receptor signaling pathway [IDA, IMP, TAS]
Gene Ontology Molecular Function
SMAD6
Gene Ontology Biological Process
- BMP signaling pathway [IDA, TAS]
- cell-substrate adhesion [IMP]
- fat cell differentiation [IDA]
- immune response [IMP]
- intracellular signal transduction [IDA, IMP]
- negative regulation of BMP signaling pathway [IDA]
- negative regulation of SMAD protein complex assembly [IDA]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IDA, IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP]
- response to laminar fluid shear stress [IEP]
- zygotic specification of dorsal/ventral axis [IMP]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA, IMP]
- type I activin receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
- ubiquitin protein ligase binding [IPI]
- I-SMAD binding [IPI]
- R-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity [IDA, IMP]
- type I activin receptor binding [IDA]
- type I transforming growth factor beta receptor binding [IDA]
- ubiquitin protein ligase binding [IPI]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
USP15 targets ALK3/BMPR1A for deubiquitylation to enhance bone morphogenetic protein signalling.
Protein kinase ALK3/BMPR1A mediates bone morphogenetic protein (BMP) signalling through phosphorylation and activation of SMADs 1/5/8. SMAD6, a transcriptional target of BMP, negatively regulates the BMP pathway by recruiting E3 ubiquitin ligases and targeting ALK3 for ubiquitin-mediated degradation. Here, we identify a deubiquitylating enzyme USP15 as an interactor of SMAD6 and ALK3. We show that USP15 enhances BMP-induced phosphorylation of ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
SMAD6 USP15 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
SMAD6 USP15 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID