An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Polyubiquitylation of AMF requires cooperation between the gp78 and TRIM25 ubiquitin ligases.

Wang Y, Ha SW, Zhang T, Kho DH, Raz A, Xie Y

gp78 is a ubiquitin ligase that plays a vital role in endoplasmic reticulum (ER)-associated degradation (ERAD). Here we report that autocrine motility factor (AMF), also known as phosphoglucose isomerase (PGI), is a novel substrate of gp78. We show that polyubiquitylation of AMF requires cooperative interaction between gp78 and the ubiquitin ligase TRIM25 (tripartite motif-containing protein 25). While TRIM25 mediates the ... [more]

Oncotarget Apr. 30, 2014; 5(8);2044-51 [Pubmed: 24810856]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AMFR GPI	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Wang Y (2014)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GPI

Gene Ontology Biological Process

Gene Ontology Molecular Function

glucose-6-phosphate isomerase activity [TAS]

Gene Ontology Cellular Component

AMFR