BAIT

PSMD13

HSPC027, Rpn9, S11, p40.5
proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Homo sapiens
PREY

RPE65

BCO3, LCA2, RP20, mRPE65, rd12, sRPE65
retinal pigment epithelium-specific protein 65kDa
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rescue of enzymatic function for disease-associated RPE65s containing various missense mutations in non-active sites.

Li S, Izumi T, Hu J, Jin HH, Siddiqui AA, Jacobson SG, Bok D, Jin M

Over 70 different missense mutations, including a dominant mutation, in RPE65 retinoid isomerase are associated with distinct forms of retinal degeneration; yet the disease mechanisms for most of these mutations have not been studied. Although some mutations have been shown to abolish enzyme activity, the molecular mechanisms leading to the loss of enzymatic function and retinal degeneration remain poorly understood. ... [more]

J. Biol. Chem. May. 21, 2014; 0(0); [Pubmed: 24849605]

Throughput

  • Low Throughput

Curated By

  • BioGRID