Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

UBE2E ubiquitin-conjugating enzymes and ubiquitin isopeptidase Y regulate TDP-43 ubiquitinylation.

Hans F, Fiesel FC, Strong JC, Jaeckel S, Rasse TM, Geisler S, Springer W, Schulz JB, Voigt A, Kahle PJ

TDP-43 characterizes insoluble protein aggregates in distinct subtypes of frontotemporal lobar degeneration (FTLD-TDP) and amyotrophic lateral sclerosis (ALS). TDP-43 mediates many RNA processing steps within distinct protein complexes. Here we identify novel TDP-43 protein interactors in a yeast 2-hybrid screen using an adult human brain cDNA library. We confirmed the TDP-43 interaction of seven hits by co-immunoprecipitation and assessed their ... [more]

J. Biol. Chem. May. 13, 2014; 0(0); [Pubmed: 24825905]

Throughput

  • Low Throughput

Curated By

  • BioGRID