BAIT

PJA1

praja-1, RP23-414J22.4
praja ring finger 1, E3 ubiquitin protein ligase
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

UBC

HMG20
ubiquitin C
GO Process (75)
GO Function (3)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination.

Lorick KL, Jensen JP, Fang S, Ong AM, Hatakeyama S, Weissman AM

A RING finger-containing protein (AO7) that binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination was identified. Mutations of cation-coordinating residues within AO7's RING finger abolished ubiquitination, as did chelation of zinc. Several otherwise-unrelated RING finger proteins, including BRCA1, Siah-1, TRC8, NF-X1, kf-1, and Praja1, were assessed for their ability to facilitate E2-dependent ubiquitination. In all cases, ubiquitination ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 28, 1999; 96(20);11364-9 [Pubmed: 10500182]

Throughput

  • Low Throughput

Additional Notes

  • E2: Ube2d2
  • likely ubiquitin conjugate

Curated By

  • BioGRID