VAMP8
Gene Ontology Biological Process
- autophagic vacuole fusion [IMP]
- endocytosis [IBA]
- eosinophil degranulation [IMP]
- exocytosis [IBA]
- membrane organization [TAS]
- mucus secretion [IMP]
- negative regulation of secretion by cell [IDA]
- neutrophil degranulation [IMP]
- positive regulation of histamine secretion by mast cell [IMP]
- post-Golgi vesicle-mediated transport [TAS]
- regulation of protein localization to plasma membrane [IDA]
- vesicle fusion [IBA]
- viral entry into host cell [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IDA]
- azurophil granule membrane [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- early endosome [TAS]
- extracellular vesicular exosome [IDA]
- late endosome membrane [IDA]
- lysosomal membrane [IDA]
- membrane [IDA]
- mucin granule [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA, TAS]
- recycling endosome [IDA]
- secretory granule membrane [IDA, TAS]
- vesicle [IDA]
USP8
Gene Ontology Biological Process
- cell proliferation [TAS]
- endosome organization [IMP]
- mitotic cytokinesis [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein K48-linked deubiquitination [IDA]
- protein K63-linked deubiquitination [IDA]
- protein deubiquitination [IMP]
- regulation of proteasomal protein catabolic process [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Dynamic regulation of ubiquitylation and deubiquitylation at the central spindle during cytokinesis.
During cytokinesis, the central spindle, a bundle of interdigitated anti-parallel microtubules between separating chromosomes, recruits various cytokinetic regulator proteins to the cleavage region. Here, we show that the level of protein ubiquitylation is strikingly and transiently elevated in Aurora B kinase-positive double-band regions of the central spindle during cytokinesis. Two deubiquitylating enzymes UBPY and AMSH, which act on endosomes in ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID