BAIT

BNI1

PPF3, SHE5, formin BNI1, L000000190, YNL271C
Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1
Saccharomyces cerevisiae (S288c)
PREY

BUD14

protein phosphatase regulator BUD14, YAR014C
Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

S. cerevisiae Kelch Proteins and Bud14 Form a Stable 520 kDa Formin-regulatory Complex that Controls Actin Cable Assembly and Cell Morphogenesis.

Gould CJ, Chesarone-Cataldo M, Alioto SL, Salin B, Sagot I, Goode BL

Formins perform essential roles in actin assembly and organization in vivo, but also require tight regulation of their activities to produce properly functioning actin structures. S. cerevisiae Bud14 is one member of an emerging class of formin regulators that target the FH2 domain to inhibit actin polymerization, but little is known about how these regulators are themselves controlled in vivo. ... [more]

J. Biol. Chem. May. 14, 2014; 0(0); [Pubmed: 24828508]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: cytokinesis (APO:0000071)

Additional Notes

  • double mutants resulted in a striking increase in the cytokinesis defective chained phenotype compared to bni1 single mutants

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BUD14 BNI1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
559193
BNI1 BUD14
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
348644

Curated By

  • BioGRID