BAIT
PARK2
PRKN
Parkinson disease (autosomal recessive, juvenile) 2, parkin
GO Process (48)
GO Function (21)
GO Component (21)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- aggresome assembly [ISO]
- cellular protein catabolic process [ISO]
- cellular response to toxic substance [ISO]
- dopamine metabolic process [IMP]
- dopamine uptake involved in synaptic transmission [IMP]
- learning [IMP]
- locomotory behavior [IMP]
- mitochondrion degradation [ISO]
- negative regulation of actin filament bundle assembly [ISO]
- negative regulation of cell death [ISO]
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [ISO]
- negative regulation of glucokinase activity [ISO]
- negative regulation of insulin secretion [ISO]
- negative regulation of neuron apoptotic process [ISO]
- negative regulation of neuron death [ISO]
- negative regulation of oxidative stress-induced neuron death [ISO]
- negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of reactive oxygen species metabolic process [ISO]
- negative regulation of release of cytochrome c from mitochondria [ISO]
- norepinephrine metabolic process [IMP]
- positive regulation of DNA binding [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of mitochondrial fusion [ISO]
- positive regulation of proteasomal protein catabolic process [ISO]
- positive regulation of protein linear polyubiquitination [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of tumor necrosis factor-mediated signaling pathway [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO]
- protein K48-linked ubiquitination [ISO]
- protein K63-linked ubiquitination [ISO]
- protein autoubiquitination [ISO]
- protein metabolic process [IMP]
- protein monoubiquitination [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IDA, ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO]
- regulation of autophagy [ISO]
- regulation of mitochondrion organization [ISO]
- regulation of neurotransmitter secretion [IMP]
- regulation of reactive oxygen species metabolic process [ISO]
- regulation of synaptic transmission [IMP]
- response to endoplasmic reticulum stress [ISO]
- response to unfolded protein [ISO]
- startle response [IMP]
- synaptic transmission, dopaminergic [IMP]
- synaptic transmission, glutamatergic [IMP]
Gene Ontology Molecular Function- F-box domain binding [ISO]
- G-protein coupled receptor binding [ISO]
- Hsp70 protein binding [ISO]
- PDZ domain binding [ISO]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- tubulin binding [ISO]
- ubiquitin binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- ubiquitin-specific protease binding [ISO]
- F-box domain binding [ISO]
- G-protein coupled receptor binding [ISO]
- Hsp70 protein binding [ISO]
- PDZ domain binding [ISO]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- tubulin binding [ISO]
- ubiquitin binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- Golgi membrane [ISO]
- LUBAC complex [ISO]
- Parkin-FBXW7-Cul1 ubiquitin ligase complex [ISO]
- aggresome [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic vesicle [ISO]
- cytosol [ISO]
- endoplasmic reticulum [ISO]
- endoplasmic reticulum membrane [ISO]
- intracellular [ISO]
- membrane raft [ISO]
- mitochondrion [ISO]
- neuron projection [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [ISO]
- postsynaptic density [ISO]
- protein complex [IDA]
- synapse [ISO]
- synaptic vesicle [ISO]
- ubiquitin ligase complex [ISO]
Mus musculus
PREY
ACTB
Actx, E430023M04Rik, beta-actin
actin, beta
GO Process (4)
GO Function (8)
GO Component (16)
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
Gene Ontology Cellular Component
- MLL5-L complex [ISO]
- NuA4 histone acetyltransferase complex [ISO]
- axon [ISO]
- blood microparticle [ISO]
- cortical cytoskeleton [IDA]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytosol [IDA]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- membrane [ISO]
- myelin sheath [IDA]
- nuclear chromatin [ISO]
- postsynaptic density [ISO]
- protein complex [ISO]
- ribonucleoprotein complex [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
BECN1 is involved in the initiation of mitophagy: It facilitates PARK2 translocation to mitochondria.
The autophagy protein BECN1/Beclin 1 is known to play a central role in autophagosome formation and maturation. The results presented here demonstrate that BECN1 interacts with the Parkinson disease-related protein PARK2. This interaction does not require PARK2 translocation to mitochondria and occurs mostly in cytosol. However, our results suggest that BECN1 is involved in PARK2 translocation to mitochondria because loss ... [more]
Autophagy Jun. 01, 2014; 10(6);1105-19 [Pubmed: 24879156]
Throughput
- Low Throughput
Curated By
- BioGRID