BAIT

UBC

HMG20
ubiquitin C
GO Process (75)
GO Function (3)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

UBR1

JBS
ubiquitin protein ligase E3 component n-recognin 1
GO Process (2)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Werner helicase-interacting protein 1 binds polyubiquitin via its zinc finger domain.

Bish RA, Myers MP

DNA repair is regulated on many levels by ubiquitination. In order to identify novel connections between DNA repair pathways and ubiquitin signaling, we used mass spectrometry to identify proteins that interact with lysine 6-linked polyubiquitin chains. From this proteomic screen, we identified the DNA repair protein WRNIP1 (Werner helicase-interacting protein 1), along with nucleosome assembly protein 1, as novel ubiquitin-interacting ... [more]

J. Biol. Chem. Aug. 10, 2007; 282(32);23184-93 [Pubmed: 17550899]

Throughput

  • High Throughput

Additional Notes

  • #LPPI
  • Likely protein-protein interaction
  • bait is an HA-tagged Lys-6-linked polyubiquitin chain, in which all lysine residues except Lys-6 are mutated to arginine

Curated By

  • BioGRID